Vesiculation pathway of clathrin-mediated endocytosis

报告题目： Vesiculation pathway of clathrin-mediated endocytosis

报  告 人：马锐 副教授，厦门大学

报告时间：2021年11月23日，周二上午10:00-11:00

报告方式/报告地点：腾讯会议，会议号 839740225

报告邀请人：施夏清

报告摘要：

During clathrin-mediated endocytosis, a small patch of flat plasma membrane is invaginated into the cell and form a vesicle under the action of a clathrin coat. How the clathrin coat deforms the membrane remains unclear and two main hypotheses have been debated. The “constant area” hypothesis assumes that clathrin molecules initially form a flat lattice which gradually obtains intrinsic curvature and finally is bent into a spherical cap while keeping the area of the clathrin lattice constant. The alternative “constant curvature” hypothesis assumes that the intrinsic curvature of the clathrin lattice remains constant while the surface area it covers increases. In this talk, I will present our recent work on the vesiculation pathway of clathrin-mediated endocytosis. By applying the Helfrich model of membrane, we were able to calculate the membrane shape for different coating area and spontaneous curvature of the clathrin coat, as well as the corresponding free energy. We found that vesiculation could happen as long as the coating area or the spontaneous curvature is large enough. We then constructed the assembling pathway of the clathrin-coat by applying the gradient-descend method to the free energy landscape of the membrane. We found that the assembling pathway is neither the constant area model nor the constant curvature model but a combination of both. By comparing with experimental data, we found that our model fit better than the two existing models.

报告人简介：

马锐，厦门大学物理科学与技术学院副教授，博士生导师，厦门大学南强学者。研究方向为生物膜力学与细胞骨架力学，分子马达与非平衡统计力学。在Physical Review Letter，PLOS Computational Biology等高水平国际期刊发表论文10余篇，其中在PLOS Computational Biology发表的论文被收录于著名数据库Fprime1000里。