Wound Geometry Dictates Non-adhesive Gap Closure

报告题目：Wound Geometry Dictates Non-adhesive Gap Closure

报告人：黄长进教授南洋理工大学

报告时间：2023年8月3日上午9:30-10:30

报告地点：物科楼245室

报告邀请人：杨恺

报告摘要：Re-epithelialization is a fundamental process for the repair of damaged tissues during wound healing and involves coordinated collective migration of epithelial cells. To investigate the effect of wound geometry on the re-epithelialization process, we have fabricated micropatterned substrates with two-dimensional (2D) non-cell-adhesive gaps of different designs, including circular gaps of different diameters, long straight stripes of different widths and long wavy stripes of different widths and curvatures, as in vitro wound healing models. In this talk, I will elucidate how the re-epithelization process of Madin-Darby canine kidney (MDCK) cells are closely regulated by the wound geometry. We find that re-epithelization occurs through purse-string contraction of actin cables accumulated around concave edges for both circular gaps and wide long straight stripes, while gap bridging either via cell extrusion or by lamellipodium extension emerges as critical cellular and molecular mechanisms for the accelerated wavy gap closure. Our discoveries deepen the understanding of mechanobiology of cell responses to curvature and help guide development of biophysical strategies for tissue repair, plastic surgery, and better wound management.

报告人简介： Dr. Changjin Huang is currently an Assistant Professor in School of Mechanical and Aerospace Engineering (MAE) at Nanyang Technological University (NTU). He received his bachelor’s degree in Thermal Energy and Power Engineering from University of Science and Technology of China (USTC) in 2008, and then Ph.D. degree in Engineering Science and Mechanics from Pennsylvania State University in 2014. Before joining NTU, he worked as a Postdoctoral Fellow in the Department of Mechanical Engineering at Northwestern University from 2014-2015, and then as a Postdoctoral Research Associate in the Department of Biomedical Engineering at Carnegie Mellon University (CMU) from 2016-2018. Dr. Huang’s research interests generally lie at the intersection of mechanics, materials, engineering and biology, including mechanics at nano-bio interface, mechanobiology, and mechanics of cells and soft materials, biofabrication, etc. Dr. Huang is an Associate Editor of Journal of Micromechanics and Molecular Physics (JMMP) and the Extreme Mechanics Letters – Early Career Advisory Board (EML-ECAB) member.